Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 1. In a crude membrane-enriched fraction, insulin-dependent diabetes results in no change in cardiac GLUT12 protein content, despite decreased cardiac GLUT4. Top panel: Western blot of cardiac muscle from healthy (Con) and type 1 diabetic (Dx) mice. Total calsequestrin (CASQ) protein content was used as a loading control. Bottom panel: Mean±SE total GLUT protein content in a crude membrane-enriched fraction, (n=5–10/group), *Pb0.05 vs. control group.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Membrane, Western Blot, Control

Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 4. Insulin stimulation causes significant translocation of GLUT4, but not GLUT12, to cell peripherals, as visualized by confocal laser scanning microscopy. Adult rat cardiac myocytes were incubated without (A, C) or with insulin (B, D, 100 μU/ml) for 30 min prior to immunofluorescent staining. Results were analyzed from three independent exper- iments with at least 70 cells/treatment. Scale bar: 10 μm.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy, Incubation, Staining

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of peritoneal macrophages on the levels of adiponectin ( ACDC ), GLUT1 , and GLUT4 transcripts in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques:

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of cytokines on the regulation of the ACDC, GLUT1, and GLUT4 genes in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Cell Culture

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Effects of recombinant TNF-α, IL-6, and IL-1β on GLUT4 protein expression. Fully differentiated adipocytes were incubated in medium containing recombinant mouse TNF-α (2 ng/ml), IL-6 (10 ng/ml), or IL-1β (20 ng/ml) for 2 days. Cells were fixed and immunostained for GLUT1 or GLUT4 expression using flow cytometry as described in Materials and Methods. The data are presented as means ± standard errors of the means for 3 to 6 independent replicates per treatment. Different letters indicate a significant difference. A P-value of <0.05 was considered significant.

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Recombinant, Expressing, Incubation, Flow Cytometry